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KMID : 0613820090190060836
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Journal of Life Science
2009 Volume.19 No. 6 p.836 ~ p.838
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Study on tPA-mediated MMP Regulation in Rat Neuronal Cells
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Ko Woon-Chul
Lee Sun-Ryung
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Abstract
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Tissue plasminogen activator (tPA) is very useful for dissolving the clots of blood, however, the use of tPA is limited to only 3-5% of ischemic stroke patients because of the narrow therapeutic time windows and negative side effects. Previous evidences suggest that limitation of tPA in thrombolytic therapy may be related to the upregulation of MMPs. However, little is known about the regulatory mechanism. In this study, we examined the role of tPA on MMP upregulation in rat neuronal cells. tPA (5 ㎍/㎖) increased MMP-9 levels of neuronal cells in a time dependent manner. Hypoxia/reoxygenation amplified tPA-induced MMP-9 levels significantly. Pretreatment with JNK inhibitor SP600125 reduced the MMP-9 response. These results suggest that tPA can upregulate MMPs in neuronal cells and that JNK kinase may be involved.
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KEYWORD
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Tissue plasminogen activator, matri, metalloproteinase, stroke, thrombolytic therapy
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